Industry-Standard Biotech Labs, Flexible for the Classroom
Explore our modular molecular biology workflow. Designed to be flexible with opportunities to partner toward your school’s or district’s NGSS and CTE-aligned goals
SECTION 1 : THE CONNECTED WORKFLOW
Primer Design / Bioinformatics & PCR
Target DNA sequence analysis
Primer design
Targeted amplification (PCR)
Our labs follow a real-world biotechnology pipeline. Teachers can present the sequence as a cohesive, multi-week research project or select individual modules to complement existing units.
DNA Cloning & DNA Analysis
DNA purification
DNA quantification
DNA gel electrophoresis
Restriction digest
Plasmid construction
Bacterial Transformation & Protein Expression
Electroporation Transformation
Cell selection & Isolation
Heat-shock Transformation
Induced protein expression
Protein Expression & Protein Analysis
Extraction and purification of expressed proteins
Protein quantification
Protein gel electrophoresis
Functional protein analysis
SECTION 2 : SAMPLE PROTOCOL PREVIEW
DNA Transformation & Bacterial Culture
Overview: Introduce plasmid DNA containing a target gene into E.coli host cells to demonstrate genetic transformation
Grade Level: 9-12 (Biology, Honors, AP, CTE Biotech)
Time Required: Two, 45 minute class periods + incubation
Core Skills: Aseptic technique, heat-shock (or electroporation) transformation, micropipetting, streak plating
Included in Kit: Competent cells, plasmid DNA, transformation buffer, heat incubator (heat-shock method) or electroporation equipment (electroporation method), agar plates, incubation equipment
Induced Protein Expression & Analysis
Overview: Induce, extract, and analyze target protein expression using SDS-PAGE gel electrophoresis
Grade Level: 10-12 (Advanced Biology, AP, CTE Biotech)
Time Required: Three, 45-minute class periods + incubation
Core Skills: Protein induction, cell lysis, SDS-PAGE gel loading/running, protein staining/visualization
Included in Kit: Cell cultures, induction reagents, gel rigs, precast gels, stain, protein ladder
SECTION 3 : REQUEST FULL TEACHER PACKET
Complete student protocols, teacher companion guides, and prep checklists are available upon request from partner districts, schools, and teachers
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