Industry-Standard Biotech Labs, Flexible for the Classroom

Explore our modular molecular biology workflow. Designed to be flexible with opportunities to partner toward your school’s or district’s NGSS and CTE-aligned goals

SECTION 1 : THE CONNECTED WORKFLOW

Primer Design / Bioinformatics & PCR

  • Target DNA sequence analysis

  • Primer design

  • Targeted amplification (PCR)

Our labs follow a real-world biotechnology pipeline. Teachers can present the sequence as a cohesive, multi-week research project or select individual modules to complement existing units.

DNA Cloning & DNA Analysis

  • DNA purification

  • DNA quantification

  • DNA gel electrophoresis

  • Restriction digest

  • Plasmid construction

Bacterial Transformation & Protein Expression

  • Electroporation Transformation

  • Cell selection & Isolation

  • Heat-shock Transformation

  • Induced protein expression

Protein Expression & Protein Analysis

  • Extraction and purification of expressed proteins

  • Protein quantification

  • Protein gel electrophoresis

  • Functional protein analysis

SECTION 2 : SAMPLE PROTOCOL PREVIEW

DNA Transformation & Bacterial Culture

Overview: Introduce plasmid DNA containing a target gene into E.coli host cells to demonstrate genetic transformation

Grade Level: 9-12 (Biology, Honors, AP, CTE Biotech)

Time Required: Two, 45 minute class periods + incubation

Core Skills: Aseptic technique, heat-shock (or electroporation) transformation, micropipetting, streak plating

Included in Kit: Competent cells, plasmid DNA, transformation buffer, heat incubator (heat-shock method) or electroporation equipment (electroporation method), agar plates, incubation equipment

Induced Protein Expression & Analysis

Overview: Induce, extract, and analyze target protein expression using SDS-PAGE gel electrophoresis

Grade Level: 10-12 (Advanced Biology, AP, CTE Biotech)

Time Required: Three, 45-minute class periods + incubation

Core Skills: Protein induction, cell lysis, SDS-PAGE gel loading/running, protein staining/visualization

Included in Kit: Cell cultures, induction reagents, gel rigs, precast gels, stain, protein ladder

SECTION 3 : REQUEST FULL TEACHER PACKET

Complete student protocols, teacher companion guides, and prep checklists are available upon request from partner districts, schools, and teachers

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